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KMID : 1098420060140040212
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Korean Journal of Medicinal Crop Science
2006 Volume.14 No. 4 p.212 ~ p.216
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Isolation of Antioxidative Compound from Scutellaria baicalensis G.
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Kim Seok-Chang
Ahn Kun-Seok
Jeon Byeong-Seon
Lee Jong-Tae
Park Wan-Jong
Park Chae-Kyu
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Abstract
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Root of Scutellaria baicalensis G. was extracted with methanol and water to give the yield of 30.0% in order to find the antioxidant substance. The extract was fractionated with diethyl ether, n-butanol and water to test the inhibitive activity against xanthine oxidase. Three fractions inhibited the activities of xanthine oxidase by 48.2%, 10.2% and 2.8%, respectively, at the amount of 0.1;{mu}g. A component that exhibited strong inhibition of xanthine oxidase was isolated from diethyl ether fraction (SE Fr.) by silica gel column chromatography and HPLC, and then identified by ^1H-NMR, ^{13}C-NMR and MS spectrophotometry. EDA (Electron Donating ability) of the compound was 28.5% at the concentration 100;{mu}g/3;ml. That was identified to be 3,5,7-trihydroxy-2’’-methoxyflavanone by spectrophotometric analysis using ^1H-NMR,;^{13}C-NMR and Mass spectrophotometry.
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KEYWORD
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Scutellaria baicalensis G, xanthine oxidase, 3, 5, 7-trihydroxy-2’-methoxyflavanone
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